Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological DiagnosticsLidija Malic 1, Abdelrahman Elmanzalawy 1, Jamal Daoud 2, Matthias Geissler 1, Alex Boutin 1, Ljuboje Lukic 1, Mojra Janta 1, Dillon Da Fonte 1, Christina Nassif 1, Teodor Veres 1
1Life Sciences Division, National Research Council of Canada, 2Galenvs Sciences, Inc.
Epigenetic markers are used for white blood cell (WBC) subtyping through the quantification of DNA methylation patterns. This protocol presents a multiplex droplet polymerase chain reaction (mdPCR) method using a thermoplastic elastomer (TPE)-based microfluidic device for droplet generation allowing for precise and multiplex methylation-specific target quantification of WBC differential counts.
